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CHEN Liangyi

  • Professor, Doctoral Advisor
  • Boya Distinguished Professor
    Deputy Director, National Center for Biomedical Imaging Science, Peking University
    Part-time researcher, McGovern Institute for Brain Research, Peking University, Beijing Collaborative Innovation Research Institute
    Head of the multimodal cross-scale biomedical Imaging Facility
    Chief scientist of National Key Research and Development Program of Ministry of Science and Technology
    Chief scientist, Major Research Program Integration Project, National Natural Science Foundation of China
  • 个人简介
    His main research direction is the abnormal mechanism of insulin secretion in the pathogenesis of diabetes, and he has invented a series of visualization means of high spatial and temporal resolution biomedical imaging. His main scientific inventions and achievements are as follows:
    1) high-resolution miniaturized two-photon microscope, which was the first to observe the morphological and functional changes of single synapses in free-behaved mice, which was considered as a "revolutionary" new tool by Professor Edvard Moser, winner of the Nobel Prize in Medicine in 2014, and his work was selected as "Top 10 Progress in Science in China in 2017" and "Nature Methods: Method of the Year 2018";
    2) the essence of reconstruction artifacts in structured light microscope at low signal-to-noise ratio was proposed for the first time, and the hidden structured light super-resolution microscope was invented, which achieved high-fidelity super-resolution imaging with phototoxicity reduced by three orders of magnitude at the maximum, and saw mitochondrial inner cristae and insulin vesicle fusion pores in living cells, which was selected as "Top 10 Progress in Optics in China in 2018";
    3) living cell dual-mode super-resolution microscope, which observed the panoramic map of organelle interaction and the new organelle black vacuol body;
    4) Proposed the concept of live cell super-resolution pathology, using live cell super-resolution imaging to predict the clinical phenotype of PMD and screen out accurate symptomatic drugs:
    5) Proposed that fluorescence signals have sparse and continuous universal priors, and invented fluorescence computing super-resolution imaging based on new mathematical principles, which is different from the traditional super-resolution methods based on specific physical principles or special fluorescent probes, achieving the highest resolution (60nm), fastest speed (564Hz) and longest imaging time (>1 hour) in live cell imaging, and won the highest prize of the final of "2021 Top 10 Social Influence Events in the field of Optics in China (Light10)" and "The First National Disruptive Technology Innovation Contest".
    The new imaging technology developed by us has promoted the basic research work of biomedical science of many collaborators at home and abroad, and has cooperated with nearly 100 groups from 60 research institutions at home and abroad, and has published many papers. On the other hand, we have transformed the original technology into high-end microscope products that are urgently needed in China, which solves the problem that high-end microscope products in China are "blocked" by foreign manufacturers. As two companies focused on biomedical imaging technology founded by the founders, the sales of commercialized instruments have exceeded 100 million yuan, and the products are sold to China, Europe and the United States, making biomedical imaging technology simpler and easier to use.
    Video version of the lab introduction: https://mp.weixin.qq.com/s/fU-XKZs_op1fZBYwU2IlZg
  • 所授课程
    Advanced Biophysics (Compulsory, 2 credits)
    Bioluminescence Imaging (Elective, 2 credits)
    Islet Cell Biology (Elective, 3 credits)
  • 获奖及荣誉
    National Outstanding Young Scholar
  • 教育背景
    1998.9-2001.4 Ph. D. Biomedical Engineering, Huazhong University of Science and Technology. Mentor: Professor Kang Huaguang and Professor Zou Shoubin
    1995.9-1998.7 Master of Bioelectronics, Department of Automation, Huazhong University of Science and Technology. Mentor: Professor Kang Huaguang and Professor Qu Anlian
    1992.9-1995.7 Xi 'an Jiaotong University, Biomedical engineering, BE
    1990.9-1992.7 Xi 'an Jiaotong University Junior Class 01
  • 工作简历
    2020.1 - Now Boya Distinguished Professor, Peking University
    2010.6 - Now Principal Investigator, IMM, Peking University, Peking University
    2004.6-2010.6 Associate Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing
    2001.4-2004.6 Postdoc fellow, University of Washington, Seattle (Advisor: Bertil Hille)
  • 团队成员
    Associate Researcher: Guo Changliang
    Secretary: Sun Tong
  • 承担项目
    National Key Research and Development Plan of the Ministry of Science and Technology Key special "Frontier Biotechnology"
    Major Instrument Development Project of National Natural Science Foundation of China (Free application)
    National Natural Science Foundation of China Outstanding Youth Foundation
    Basic Science Center, National Natural Science Foundation of China
    Key project of Beijing Natural Science Foundation
  • 代表性论文及论著
    1. Ren H, Li Y, Han C, Yu Y, Shi B, Peng X, Zhang T, Wu S, Yang X, Kim S, Chen L*, Tang C*. Pancreatic α and β cells are globally phase-locked. Nat Commun., 2022 Jun 28;13(1):3721.
    2. Zhao W, Zhao S, Li L, Huang X, Xing S, Zhang Y, Qiu G, Han Z, Shang Y, Sun D, Shan C, Wu R, Gu L, Zhang S, Chen R, Xiao J, Mo Y, Wang J, Ji W, Chen X, Ding B, Liu Y, Mao H, Song B, Tan J, Liu J, Li H*, Chen L*. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy, Nat Biotech., 2022 Apr;40(4):606-617.
    "Technical advance", In Faculty Opinions, Dec 2 2021; 10.3410/f.741163644.793590090
    https://www.youtube.com/watch?v=8hmQ-LAdpfM
    3. Zong W, Wu R*, Chen S, Wu J, Wang H, Zhao Z, Chen G, Tu R, Wu D, Hu Y, Xu Y, Wang Y, Duan Z, Wu H, Zhang Y, Zhang Y, Wang A*, Chen L*, Cheng H. Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging, Nat Methods. 2021 Jan;18(1):46-49.
    4. Zheng X, Duan R, Li L, Xing S, Ji H, Yan H, Gao K, Wang J, Wang J*, Chen L*. Live-cell superresolution pathology reveals different molecular mechanisms of Pelizaeus-Merzbacher disease, Science Bulletin, 30 December 2020; 65(24): 2061-2064.
    5. Dong D, Huang X, Li L, Mao H, Mo Y, Zhang G, Zhang Z, Shen J, Liu W, Wu Z, Liu G, Liu Y, Yang H, Gong Q, Shi K*, Chen L*, Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome, Light Sci Appl. 2020 Jan 28;9:11.
    6. Zhao J, Zong W, Zhao Y, Gou D, Liang S, Shen J, Wu Y, Zheng X, Wu R, Wang X, Niu F, Wang A, Zhang Y, Xiong JW, Chen L*, Liu Y*. In Vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development. Elife. 2019 Jan 29;8. pii: e41540. Highlighted in the Nature Reviews Endocrinology (2019).
    7. Huang X, Fan J, Li L, Liu H, Wu R, Wu Y, Wei L, Mao H, Lal A, Xi P, Tang L, Zhang Y, Liu Y, Tan S*, Chen L*. Fast, long-term super-resolution imaging with Hessian structured illumination microscopy, Nat Biotech., 2018 Jun;36(5):451-459. doi: 10.1038/nbt.4115.
    Highlighted in the Nature Methods (2018).
    https://www.youtube.com/watch?v=U9Om7DPxzd0&feature=youtu.be
    8. Zong W, Wu R, Li M, Hu Y, Li Y, Li J, Rong H, Wu H, Xu Y, Lu Y, Jia H, Fan M, Zhou Z, Zhang Y*, Wang A*, Chen L*, Cheng H. Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving Mice. Nat Methods. 2017 Jul;14(7):713-719.
    An exclusive commentary in Light: Science & Applications (2017).
    2017年中国科学十大进展，2017年中国生命科学十大进展，2017年中国十大医学科技新闻；“Nature Methods: Method of the Year 2018”; https://www.youtube.com/watch?v=AED0SJVAlp0
    9. Yuan T, Liu L, Zhang Y, Wei L, Zhao S, Zheng X, Huang X, Boulanger J, Gueudry C, Lu J, Xie L, Du W, Zong W, Yang L, Salamero J, Liu Y*, Chen L*. Diacylglycerol Guides the Hopping of Clathrin-Coated Pits along Microtubules for Exo-Endocytosis Coupling. Dev Cell. 2015 Oct 12;35(1):120-30.
    Previewed in the same issue of Dev Cell, highlighted in the F1000Prime as NEW FINDING and INTERESTING HYPOTHESIS and also highlighted on the American Society of Cell Biology (ASCB) website.
    10. Zong W, Zhao J, Chen X, Lin Y, Ren H, Zhang Y, Fan M, Zhou Z, Cheng H, Sun Y*, Chen L*. Large-field high-resolution two-photon digital scanned light-sheet microscopy. Cell Res. 2015 Feb;25(2):254-7.