• Assistant Professor, Principal Investigator
• College of Future Technology
  National Biomedical Imaging Center

• 个人简介

  Our lab focuses on advancing and integrating cutting-edge cryo-electron microscopy (cryo-EM) methods to achieve high-resolution structural analysis of in situ and semi-in situ macromolecular complexes, exploring how molecular machines are organized and function within their native cellular environments. We are particularly interested in the spectrin–actin membrane skeleton and associated membrane domains, aiming to reveal novel higher-order organizations and previously unrecognized interaction networks of the involved membrane-associated protein complexes. By integrating cryo-EM with biochemistry, cell biology, optical imaging, and computational biology methods, we ultimately seek to bridge molecular structure with physiological function and disease mechanisms.

• 教育背景

  2010-2015 Ph.D. in Structural Biology, School of life science, Tsinghua University
  2006-2010 B.S. in Biochemistry, School of Ocean Life Science, Ocean University of China
  

• 工作简历

  2025-Now Assistant Professor, College of Future Technology, Peking University
  2023-2025 Research Professor, School of life science, Peking University
  2018-2025 Research Associated Professor, School of life science, Peking University
  2016-2018 Post-doctoral Fellow, School of life science, Peking University
  

• 代表性论文及论著

  1. Li, N.#*, Chen, S.#, Xu, K., He, M., Dong, M., Zhang, Q., Gao, N.* (2023) Structural basis of membrane skeleton organization in red blood cells. Cell 186, 1912-1929.
  2. Wang, X.#, Ma, L., Li, N.*, Gao, N.* (2023). Structural insight into the assembly and mechanism of monkeypox virus DNA polymerase complex F8-A22-E4-H5. Molecular Cell 83(23):4398-4412
  3. Li, N.#, Gao, Y., Zhang, Y., Yu, D., et al., Tye, B. K.*, Li, Q.*, Gao, N.*, Zhai, Y.* (2024) Parental histone transfer caught at the replication fork. Nature 627, 890-897.
  4. Liu, S.#, Jiang, T.#, Bu, F.#, Zhao, J.#, Wang, G.#, Yang, G., Kong, J., Qie, Y., Wen, P., Fan, L., Li, N.*, Gao, N.*, and Qiu, X.* (2024). Molecular mechanisms underlying the BIRC6-mediated regulation of apoptosis and autophagy. Nature Communications 15.
  5. Cheng, J.#, Li, N.#, Huo, Y., Dang, S., Tye, B.K., Gao, N., and Zhai, Y. (2022). Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase. Nature Communications 13.
  6. Cheng, J.#, Li, N.#*, Wang, X., Hu, J., Zhai, Y., Gao, N.*. (2020). Structural insight into the assembly and conformational activation of human origin recognition complex. Cell Discovery 6, 88.
  7. Jiang, F.#, Li, N.#, Wang, X.#, Cheng, J.#, Huang, Y., Yang, Y., Yang, J., Cai, B., Wang, Y.P., Jin, Q.*, and Gao, N.* (2019). Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell 177, 370-83.
  8. Li, N.#, Lam, W.H.#, Zhai, Y.#*, Cheng, J.#, Cheng, E., Zhao, Y., Gao, N.*, and Tye, B.K.* (2018). Structure of the origin recognition complex bound to DNA replication origin. Nature 559, 217-222.
  9. Li, N.#, Wu, J.X.#, Ding, D., Cheng, J., Gao, N.*, and Chen, L.* (2017). Structure of a Pancreatic ATP-Sensitive Potassium Channel. Cell 168, 101-110.
  10. Li, N.#, Zhai, Y.#*, Zhang, Y., Li, W., Yang, M., Lei, J., Tye, B.K.*, and Gao, N.* (2015). Structure of the eukaryotic MCM complex at 3.8 A. Nature 524, 186-191.
  11. Ge, J.#, Li, W.#, Zhao, Q.#, Li, N.#, Chen, M., Zhi, P., Li, R., Gao, N.*, Xiao, B.*, and Yang, M.* (2015). Architecture of the mammalian mechanosensitive Piezo1 channel. Nature 527, 64-69.
  12. Li, N.#, Chen, Y., Guo, Q., Zhang, Y., Yuan, Y., Ma, C., Deng, H., Lei, J., and Gao, N. (2013). Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit. Nucleic Acids Res 41, 7073-7083.