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GAO Ning

  • Professor
  • Associate Dean, School of Life Sciences, Peking University
    Director, Key Laboratory of Membrane Biology, Peking University
    Center for Life Sciences, Peking University-Tsinghua University
    National Biomedical Imaging Center, Peking University
    Head of the multimodal cross-scale biomedical Imaging Facility
  • 个人简介
    The laboratory focuses on the core content of life sciences, mainly using cryo-electron microscopy technology to study the structure and function of molecular machines related to major life processes, and explore important basic biological mechanisms and the mechanism of major human diseases. In recent years, the laboratory's research work has focused on nucleic acid-protein complex molecular machines and large protein complexes with complex composition. The important research areas of the laboratory include:
    (1) The ribosome of pathogens is a natural target of many antimicrobial drugs, and human ribosome biogenesis is abnormally up-regulated in a variety of human tumor cells, and is positively correlated with the ability of tumor metastasis. The laboratory uses bacteria, yeast, and human cell lines as models to study the bioassembly mechanism of human pathogens, model organisms, and human ribosomes, the novel regulatory functions and molecular mechanisms of different ribosome-binding proteins in protein translation, and the discovery of potential drug targets.
    (2) DNA replication is the core of molecular biology, and the disorders of DNA replication are also a hallmark feature of many tumors. The structure and working mechanism of the protein molecular machine in the initiation of DNA replication in eukaryotes are studied in the laboratory.
    (3) The laboratory also works closely with a number of research groups, including clinical scientists, to study the structure and mechanism of therapeutic targets for major human diseases.
  • 获奖及荣誉
    2019 10,000 people plan, Batch No.4, Department of Central Organization & leading talents of scientific and technological innovation
    2018 Young and middle-aged scientific and technological innovation leaders, Ministry of Science and Technology
    2018 Youth Science Award for Outstanding Achievements in Scientific Research in Higher Education, Ministry of Education
    2018 Changjiang Distinguished Professor
    2017 Tan Jiazhen Life Science Innovation Award
    2017 National Natural Science Foundation Outstanding Youth Fund
    2016 Mao Yisheng Prize for Youth scientists, Beijing
    2016 Zhongyuan Union Life Medicine Innovation Breakthrough Award
    2016 Wuxi Apptec Life Chemist Award
    2014 National Natural Science Foundation of China Outstanding Youth Fund
  • 个人履历
    2017/4-present, Professor, School of Life Sciences, Peking University
    2017/4-present, Principal Investigator, Center of Life Sciences, Peking University-Tsinghua University
    2008-11-2017/3, Special Associate Research Fellow, Assistant Professor, Associate Professor (with tenure), Professor, School of Life Sciences, Tsinghua University
    2006-2008, Postdoctoral Fellow, Wadsworth Center, New York, Howard Hughes Medical Institute, Department of Biochemical and Molecular Biophysics, Columbia University
    2001-2006, Ph. D. Department of Biomedical Sciences, State University of New York at Albany
    1996-2000, Bachelor, School of Life Sciences, Peking University
  • 团队成员
    Associate researcher Li Ningning
    Secretary Liu Yang
  • 承担项目
    Key projects from the Fund committee, Jieqing projects, etc
  • 代表性论文及论著
    1. Chen, Y., Tsai, B., Li, N., and Gao, N.# (2022). Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding. Nature communications 13, 3410.
    2. Cheng, J., Li, N., Huo, Y., Dang, S., Tye, B.-K.#, Gao, N.#, and Zhai, Y.# (2022). Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase. Nature communications 13, 1396.
    3. Ma, C., Wang, C., Luo, D., Yan, L., Yang, W., Li, N., and Gao, N. (2022). Structural insights into the membrane microdomain organization by SPFH family proteins. Cell Res 32, 176-189.
    4. Yang, F., Guo, L.L., Li, Y., Wang, G.P., Wang, J., Zhang, C., Fang, G.X., Chen, X., Liu, L., Yan, X., et al. (2021). Structure, function and pharmacology of human itch receptor complexes. Nature 600, 164-169.
    5. Zheng, L., Zheng, Z., Li, X., Wang, G., Zhang, K., Wei, P., Zhao, J.#, and Gao, N#. (2021). Structural insight into the mechanism of energy transfer in cyanobacterial phycobilisomes. Nature communications 12, 5497.
    6. Zheng, L., Liu, Z., Wang, Y., Yang, F., Wang, J., Huang, W., Qin, J., Tian, M., Cai, X., Liu, X., Mo, X., Gao, N.#, and Jia, D.# (2021). Cryo-EM structures of human GMPPA-GMPPB complex reveal how cells maintain GDP-mannose homeostasis. Nat Struct Mol Biol 28, 1-12.
    7. Zhao, T., Chen, Y.M., Li, Y., Wang, J., Chen, S., Gao, N.#, and Qian, W.# (2021). Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding. Genome Biol 22, 16.
    8. Micic, J., Li, Y., Wu, S., Wilson, D., Tutuncuoglu, B., Gao, N.#, and Woolford, J.L., Jr.# (2020). Coupling of 5S RNP rotation with maturation of functional centers during large ribosomal subunit assembly. Nature communications 11, 3751.
    9. Wilson, D.M., Li, Y., LaPeruta, A., Gamalinda, M., Gao, N.#, and Woolford, J.L., Jr.# (2020). Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel. Nature communications 11, 5111.
    10. Liang, X., Zuo, M.Q., Zhang, Y., Li, N., Ma, C., Dong, M.Q., and Gao, N.# (2020). Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. Nature communications 11, 3542.
    11. Wang, W., Li, W., Ge, X., Yan, K., Mandava, C.S., Sanyal, S.#, and Gao, N.# (2020). Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation. PNAS 117, 15609-15619.
    12. Ye, Y., Wu, H., Chen, K., Clapier, C.R., Verma, N., Zhang, W., Deng, H., Cairns, B.R.#, Gao, N.#, and Chen, Z.# (2019). Structure of the RSC complex bound to the nucleosome. Science 366, 838-843.
    13. Zheng, L., Li, Y., Li, X., Zhong, Q., Li, N., Zhang, K., Zhang, Y., Chu, H., Ma, C., Li, G.#, Zhao, J.#, and Gao, N#. (2019). Structural and functional insights into the tetrameric photosystem I from heterocyst-forming cyanobacteria. Nat Plants 5, 1087-1097.
    14. Dong, D., Zheng, L., Lin, J., Zhang, B., Zhu, Y., Li, N., Xie, S., Wang, Y., Gao, N.#, and Huang, Z.# (2019). Structural basis of assembly of the human T cell receptor-CD3 complex. Nature 573, 546-552.
    15. Ma, C., Wu, X., Sun, D., Park, E., Catipovic, M.A., Rapoport, T.A.#, Gao, N.#, and Li, L.# (2019). Structure of the substrate-engaged SecA-SecY protein translocation machine. Nature communications 10, 2872.
    16. Jiang, F., Li, N., Wang, X., Cheng, J., Huang, Y., Yang, Y., Yang, J., Cai, B., Wang, Y.P., Jin, Q.#, and Gao, N.# (2019). Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell 177, 370-383 e315.
    17. Yan, L., Wu, H., Li, X., Gao, N.#, and Chen, Z. # (2019). Structures of the ISWI-nucleosome complex reveal a conserved mechanism of chromatin remodeling. Nat Struct Mol Biol 26, 258-266.
    18. Li, N., Lam, W.H., Zhai, Y.#, Cheng, J., Cheng, E., Zhao, Y., Gao, N.#, and Tye, B.K.# (2018). Structure of the origin recognition complex bound to DNA replication origin. Nature 559, 217-222.
    19. Zhai, Y.#, Li, N., Jiang, H., Huang, X., Gao, N.#, and Tye, B.K.# (2017). Unique Roles of the Non-identical MCM Subunits in DNA Replication Licensing. Molecular Cell 67, 168-179.
    20. Li, Z., Guo, Q., Zheng, L., Ji, Y., Xie, Y.-T., Lai, D.-H., Lun, Z.-R., Suo, X., and Gao, N.# (2017). Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii. Cell Research 27, 1275-1288.
    21. Peng, R., Xu, Y., Zhu, T., Li, N., Qi, J., Chai, Y., Wu, M., Zhang, X., Shi, Y., Wang, P.#, Wang, J.#, Gao, N.#, and Gao, G.F.# (2017). Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures. Cell Research 27, 853-864.
    22. Ma, C., Kurita, D., Li, N., Chen, Y., Himeno, H.#, and Gao, N.# (2017). Mechanistic insights into the alternative translation termination by ArfA and RF2. Nature 541, 550-553.
    23. Ma, C., Wu, S., Li, N., Chen, Y., Yan, K., Li, Z., Zheng, L., Lei, J., Woolford, J.L., Jr.#, and Gao, N.# (2017). Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nat Struct Mol Biol 24, 214-220.
    24. Zhai, Y., Cheng, E., Wu, H., Li, N., Yung, P.Y., Gao, N.#, and Tye, B.K.# (2017). Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer. Nat Struct Mol Biol 24, 300-308.
    25. Li, N., Wu, J.X., Ding, D., Cheng, J., Gao, N.#, and Chen, L.# (2017). Structure of a Pancreatic ATP-Sensitive Potassium Channel. Cell 168, 101-110 e110.
    26. Gu, J., Wu, M., Guo, R., Yan, K., Lei, J., Gao, N.#, and Yang, M.# (2016) The architecture of mammalian respirasome, Nature, 537, 639-643.
    27. Wu, S., Tutuncuoglu, B., Yan, K., Brown, H., Zhang, Y., Tan, D., Gamalinda, M., Yuan, Y., Li, Z., Jakovljevic, J., Ma, C., Lei, J., Dong, M.-Q., Woolford, J.L.#, and Gao, N.# (2016). Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes. Nature 534, 133-137.
    28. Zhang, D., Yan, K., Liu, G., Song, G., Luo, J., Shi, Y., Cheng, E., Wu, S., Jiang, T., Lou, J., Gao, N. #, and Qin, Y.# (2016). EF4 disengages the peptidyl-tRNA CCA end and facilitates back-translocation on the 70S ribosome. Nat Struct Mol Biol 23, 125-131.
    29. Zhang, Y., Mandava, C.S., Cao, W., Li, X., Zhang, D., Li, N., Zhang, Y., Zhang, X., Qin, Y., Mi, K., Lei, J.#, Sanyal, S.#, and Gao, N.# (2015). HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions. Nat Struct Mol Biol 22, 906-913.
    30. Ge, J., Li, W., Zhao, Q., Li, N., Chen, M., Zhi, P., Li, R., Gao, N.#, Xiao, B.#, and Yang, M.# (2015). Architecture of the mammalian mechanosensitive Piezo1 channel. Nature 527, 64-69.
    31. Li, N., Zhai, Y.#, Zhang, Y., Li, W., Yang, M., Lei, J., Tye, B.K.#, and Gao, N#. (2015). Structure of the eukaryotic MCM complex at 3.8 A. Nature 524, 186-191.
    32. Zhang, Y., Ma, C., Yuan, Y., Zhu, J., Li, N., Chen, C., Wu, S., Yu, L., Lei, J.#, and Gao, N.# (2014). Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome. Nat Struct Mol Biol 21, 1042-1046.