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  • 办公地址：

    436, LUI CHE WOO BUILDING

  • 联系电话：

    010-62766905

  • 电子邮箱：

    yulongli@pku.edu.cn

  • 个人网页：

    http://yulonglilab.org

Li Yulong

  • Professor, Principal Investigator
  • School of Life Sciences, Peking University
    McGovern Institute for Brain Research, Peking University
    Center for Life Sciences, Peking University-Tsinghua University
    National Biomedical Imaging Center, Peking University
  • 个人简介
    The human brain is composed of billions of neurons, which in turn form complex networks through trillions of synapses. Different types of neurons project far or near and communicate with other neurons through synapses to achieve advanced neural functions such as perception, decision-making and movement.
    The greatest challenge in studying the brain is its immense complexity. The lab focuses on the basic structure of neuronal communication, the synapse, and conducts research on two levels: one is to develop cutting-edge tools, namely new imaging probes, to parse the complex functions of the nervous system at both spatial and temporal scales; the other is to explore the regulatory mechanisms of synaptic transmission, especially the regulation of neurotransmitter release under physiological and pathological conditions, with the help of advanced tools.
    Specifically, for tool development, we focus on:
    1. Combining optogenetics and fluorescence imaging to noninvasively study the electrical synaptic connections between neurons. Abnormal electrical synaptic connections can lead to diseases such as deafness, epilepsy, brain tumors and heart dysfunction.
    2. Developing genetically encoded fluorescent probes for the detection of neurotransmitters/modulators, key mediators of chemical synaptic transmission between neurons, which are closely related to perception, learning and memory, and emotions.
    Using the above fluorescent probes, functional and physiological studies have focused on:
    1. Combining bioinformatics, analytical chemistry, biochemistry, physiology and imaging methods, systematically explore and identify potential new small molecule neurotransmitters.
    2. Study the proteomics of "high density core vesicles", an important secretory vesicle in neurons, and analyze the composition of neuropeptides in the vesicles. These neuropeptides have important regulatory effects on regulating food intake, aggressive behavior and biological rhythms
    3. Find the receptors for the new chemical messengers/modulatory small molecules, i.e. , to find the ligands of the "orphan" receptors.
    4. Combine two-photon imaging with genetically encoded fluorescent probes, using Drosophila and mice as model organisms, to study the mechanisms of brain function in olfactory transmission or sleep.
  • 所授课程
    Comprehensive Science Experiment Course (Compulsory, Major, 4 credits)
    Current topics on Genetics (Compulsory, Major, 2 credits)
    Advanced Neurobiology (Major, 4 credits)
    Journal Club of the Frontier for Life Sciences (Optional, 2 credits)
    Exploring the Mystery of Intracranial "Black Box" - Introduction to Brain Science (Elective, 1 credit)
    Advances in Neurobiology (Compulsory, 1 credit)
    Advanced Biophysics (Compulsory, 1 credit)
  • 获奖及荣誉
    The National Science Fund for Distinguished Young Scholars
  • 个人履历
    2020 – present Full professor with tenure, School of Life Sciences, PKU, Beijing
    2019 – 2020 Associate professor, School of Life Sciences, PKU, Beijing
    2012 – 2019 Assistant professor, School of Life Sciences, PKU, Beijing
    2006 – 2012 Post-Doctoral Fellow, Molecular and Cellular Physiology, Stanford University, Palo Alto, CA, Postdoctoral Mentor-Richard Tsien
    2000 – 2006 Ph.D., Duke University, Durham, NC, Mentor-George Augustine
    1996 – 2000 B.S., Summa Cum Laude, PKU, Beijing
  • 承担项目
    National Natural Science Foundation project, American Brain Project U01, Peak fund, etc
  • 代表性论文及论著
    1. Jing, M., Zhang, P., Wang, G., Feng, J., ... Zhu, JJ. # & Li, Y. # (2018). A genetically-encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nature Biotechnology, 36(8), 726-737.
    2. Sun, F., Zeng, J., Jing, M., Zhou, J., Feng, J., ... & Li, Y. # (2018). A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice. Cell, 174(2), 481-496.
    3. Yu, H., Zhao, T., Liu, S., Wu, Q., Johnson, O., Wu, Z., Zhuang, Z., Shi, Y., He, R., Yang, Y., Sun, J., Wang, X., Xu, H., Zeng, Z., Lei, X., Luo, W.# & Li, Y.# (2019). MRGPRX4 is a bile acid receptor for human cholestatic itch. eLife, 8, e48431.
    4. Wu, L., Dong, A., Dong, L., Wang, S. Q., & Li, Y. # (2019). PARIS, an optogenetic method for functionally mapping gap junctions. eLife, 8, e43366.
    5. Feng, J., Zhang, C., Lischinsky, JE., Jing, M., ... & Li, Y. # (2019). A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine. Neuron, 102(4), 745-761.
    6. Jing, M. #, Li, Y., Zeng, J., Huang, P., ... & Li, Y. # (2020). An optimized acetylcholine sensor for monitoring in vivo cholinergic activity. Nature Methods, 17(11), 1139-1146.
    7. Sun, F., Zhou, J., Dai, B., Qian, T., …, Lin, D. #, Cui, G. #, & Li, Y. # (2020). Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Nature Methods, 17(11), 1156-1166.
    8. Qian, C., Wu, Z., Sun, R., Yu, H., Zeng, J., Rao, Y., & Li, Y. # (2021). Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose. eLife, 10, e65417.
    9. Wan, J., Peng, W., Li, X., Qian, T., …, & Li, Y. # (2021). A genetically encoded GRAB sensor for measuring serotonin dynamics. Nature Neuroscience, 24(5), 746-752.
    10. Dong, A., He, K., Dudok, B., Farrell, J. S., …, & Li, Y. # (2022) A fluorescent sensor for spatiotemporally resolved endocannabinoid dynamics in vitro and in vivo. Nature Biotechnology, 40 (5), 787-798.
    11. Wu, Z. #, He, K., Chen, Y., Li, H., Pan, S., Li, B., Liu, T., Wang, H., Du, J., Jing, M., & Li, Y. # (2022). A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Neuron, 110, 770-782 e775.